Restriction Enzymes

Restriction Enzymes

Watch this webinar to learn all about restriction enzymes, and how the MCAT will test these “molecular scissors” in the context of DNA cloning. You will learn about DNA cloning, how to read a restriction enzyme table, and how to identify cut sites.

At a glance


Essential skills

  • Be able to read a restriction enzyme table and identify restriction enzyme cut sites

  • Be able to read a plasmid

  • Translate a gel into restriction enzyme digest results

  • Use antibiotic resistances to predict colony growth

  • Identify cloning errors


Knowledge Fundamentals

  • Definitions of vector, insert, and recombinant plasmid

  • DNA cloning


On the MCAT

  • Restriction enzymes will appear in text or in a table

  • You don't need to memorize restriction enzyme sites, but you do need to predict where they might cut


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