Restriction Enzymes

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Watch this webinar to learn all about restriction enzymes, and how the MCAT will test these “molecular scissors” in the context of DNA cloning. You will learn about DNA cloning, how to read a restriction enzyme table, and how to identify cut sites.

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At a glance

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Essential skills

Be able to read a restriction enzyme table and identify restriction enzyme cut sites
Be able to read a plasmid
Translate a gel into restriction enzyme digest results
Use antibiotic resistances to predict colony growth
Identify cloning errors

assessment

Knowledge Fundamentals

Definitions of vector, insert, and recombinant plasmid
DNA cloning

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On the MCAT

Restriction enzymes will appear in text or in a table
You don't need to memorize restriction enzyme sites, but you do need to predict where they might cut

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Restriction Enzymes

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At a glance

Glycolysis and Gluconeogenesis

Punnett Squares

Protein Chromatography

Gel Electrophoresis and Western Blot